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UNIVERSITEIT UTRECHT
UTRECHT
Unclaimed
Subcontracting experience
4 records from government sources. Past participation is not evidence of current teaming interest.
| Role | Agency | Description | Vehicle | Obligated | Action date | Period |
|---|---|---|---|---|---|---|
| Subcontractor | National Science Foundation | TRANSCRIBE AND CODE 60 COGNITIVE INTERVIEWS, ANLYSES OF THE TRANSCRIPTS,AND PROVIDE FINAL RECOMMENDATIONS. 52610 Parent award context Parent award agency: National Science Foundation · National Science Foundation Parent award NAICS: 541720 Parent award performance state: VA Parent award description: NCSES BROAD AGENCY ANNOUNCEMENT, R&D PROJECT TITLED: "IMPROVING SEXUAL ORIENTATION AND GENDER IDENTITY MEASUREMENT AMONG MONOLINGUAL SPANISH SPEAKERS" View parent award on USAspending | — | $58,950 | May 15, 2023 | — – — |
| Subcontractor | Department of Health and Human Services | TASK 1: VIRUSES OF STUDY AND GENERATION OF RECOMBINANT VIRUSES (CEIRR-CRIPT). THE RECENT IDENTIFICATION AND COLLECTION OF 2009 PANDEMIC PRECURSOR VIRUSES BY THE LAB OF PROF. DR. ADOLFO GARCIA SASTRE AT CEIRR-CRIPT ALLOWS NEW EXPERIMENTAL RESEARCH INTO PANDEMIC EMERGENCE AT THE HUMAN-SWINE INTERFACE . THE VIRUSES OF THIS COLLECTION WILL BE COMPARED TO A SET OF H1N1 AND H3N2 HUMAN VIRUSES AVAILABLE AT UU. GUIDED BY PHYLOGENETIC ANALYSES AND RESULTS FROM TASK 2-4 (UU), CEIRR-CRIPT WILL GENERATE RECOMBINANT VIRUSES TO MAKE CAUSAL LINKS BETWEEN PHENOTYPIC TRAITS TO GENETIC FEATURES (UU).
TASK 2: BIOLAYER INTERFEROMETRIC KINETIC ANALYSIS OF VIRUS-RECEPTOR INTERACTIONS (UU). THE HA-NA-RECEPTOR BALANCE OF (PRE)PANDEMIC VIRUSES WILL BE STUDIED USING BLI. WE WILL USE TAILOR-MADE RECOMBINANT GLYCOPROTEINS DEVELOPED AT UU THAT BETTER MIMIC THE RECEPTORS IAVS ENCOUNTER IN VIVO THAN THE TYPICALLY USED SYNTHETIC GLYCANS. THE GLYCOSYLATION AND SIALYLATION OF THESE RECOMBINANT SOLUBLE GLYCOPROTEINS (E.G. LAMP1 AND GLYCOPHORIN A) CAN BE MODIFIED BY CO-EXPRESSION OF ENZYMES SUCH AS ST6GAL1 (INCREASED α2,6-LINKED SIAS) AND CMAH (SYNTHESIS OF NEU5GC, ONLY PRESENT IN SWINE) AND BY THEIR EXPRESSION IN HEK293T CELL DERIVATIVES LACKING ONE OR MORE GLYCOSYLTRANSFERASES. VIRUSES WILL BE ANALYZED FOR THEIR ABILITY TO BIND SYNTHETIC GLYCANS AND RECOMBINANT BIOTINYLATED GLYCOPROTEINS. BINDING VIA HA WILL BE STUDIED IN THE PRESENCE OF NA INHIBITORS (NAI). VIRUS PARTICLE NUMBERS WILL BE DETERMINED AS THESE CO-DETERMINE THE INITIAL BINDING RATE, WHICH IS THE PRIME PHYSIOLOGICALLY RELEVANT PARAMETER OF VIRUS-RECEPTOR BINDING. NA-DEPENDENT SELF-ELUTION OF VIRUS PARTICLES WILL BE EXAMINED BECAUSE IT REFLECTS THE HA-NA-RECEPTOR BALANCE.
TASK 3: INTERACTION OF IAV WITH MUCUS (UU). THE HA-NA BALANCE IS CRUCIAL FOR MOTILITY IN MUCUS LAYERS AND REACHING OF EPITHELIAL CELLS FOR UPTAKE. MUCUS WILL BE HARVESTED FROM PRIMARY EPITHELIAL CELL CULTURES ESTABLISHED AT UU AND CHARACTERIZED USING LECTIN BINDING ASSAYS AND PROTEOMICS AND GLYCOMICS ANALYSIS VIA MASS SPEC (UU). FOR BLI ASSAYS, MUCUS WILL BE ATTACHED TO SENSORS USING DIFFERENT LECTINS AND THE INTERACTION KINETICS OF VIRUSES WILL BE ESTABLISHED. IN ADDITION, WE WILL ANALYZE THE ABILITY OF MUCUS TO INTERFERE WITH RECEPTOR BINDING TO OR DRIVE ELUTION FROM RECEPTOR-COATED SURFACES USING BLI. UU PREVIOUSLY CONFIRMED THAT THE ABILITY OF MUCUS TO INTERFERE WITH VIRUS-RECEPTOR BINDING DEPENDS ON THE PRESENCE OF SIAS IN MUCUS. THE INHIBITORY EFFECT OF MUCUS ON THE CLEAVAGE ACTIVITY OF THE NA PROTEIN WILL BE PROBED USING IN SOLUTION MUNANA CLEAVAGE ASSAYS AND/OR ENZYME-LINKED LECTIN ASSAYS. THIS WILL PROVIDE INSIGHT INTO: I) THE ABILITY OF HUMAN AND SWINE MUCUS TO INTERFERE WITH RECEPTOR INTERACTIONS OF SWINE AND HUMAN IAVS AND II) TO WHAT EXTENT THIS IS CORRELATED WITH CHARACTERISTICS OF HA, NA, AND THEIR BALANCE.
TASK 4: IMPORTANCE OF HA-NA BALANCE FOR HUMAN AND SWINE VIRUS REPLICATION IN (PRIMARY) EPITHELIAL CELLS (UU). LASTLY WE WILL ADDRESS THE INHIBITORY EFFECT OF SWINE AND HUMAN MUCUS, THE CONTRIBUTION OF THE TARGET CELL RECEPTOR-REPERTOIRE, AND THE HA-NA-RECEPTOR BALANCE ON VIRUS PROPAGATION. REPLICATION ASSAYS WILL BE CONDUCTED ON IMMORTALIZED CELLS IN THE PRESENCE/ABSENCE OF SWINE AND HUMAN MUCUS TO ESTABLISH THE SPECIES- AND MUCUS-SPECIFIC INTERFERENCE OF VIRUS REPLICATION. IN ADDITION, WE WILL STUDY REPLICATION ON SWINE AND HUMAN PRIMARY EPITHELIAL CELL CULTURES WITH VIRUSES THAT DIFFER IN THEIR HA-NA-RECEPTOR BALANCE. WE WILL PERFORM COMPETITION ASSAYS TO ELUCIDATE SUBTLER DIFFERENCES IN REPLICATIVE ABILITIES. COLLECTIVELY, THESE ASSAYS WILL ALLOW US TO DETERMINE THE CORRELATION BETWEEN I) VIRUS TROPISM AND ZOONOTIC POTENTIAL, II) IAV REPLICATION IN PRIMARY CULTURES, III) HA-NA-RECEPTOR BALANCE AND IV) THE INTERFERENCE OF MUCUS. 0258-A715-4612 Parent award context Parent award agency: Department of Health and Human Services · National Institutes of Health Parent award NAICS: 541715 Parent award performance state: NY Parent award description: MSSM CEIRR - THIS CONTRACT WILL SUPPORT THE NIAID CENTERS OF EXCELLENCE FOR INFLUENZA RESEARCH AND RESPONSE. View parent award on USAspending | — | $326,313 | Mar 2, 2023 | — – — |
| Subcontractor | General Services Administration | GSA EPA -21F0015 22657 RO1 Parent award context Parent award agency: General Services Administration · Federal Acquisition Service Parent award NAICS: 541611 Parent award performance state: DC Parent award description: EPA BPA TASK ORDER 03 AGAINST ID11210010 View parent award on USAspending | GS00F010CAUSAspending | $79,533 | Feb 16, 2022 | — – — |
| Subcontractor | Environmental Protection Agency | EPA ENERGY STAR C&I BPA 19KABO0046 RO2 CO2 · Prime awardUSAspending | 68HE0H18A0003USAspending | $8,000 | Nov 11, 2021 | — – — |
Contract vehicles are shown only when present in the source record. Linked award numbers and vehicles open the authoritative record on USAspending.gov.