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UNIVERSITY OF THE WESTERN CAPE
BELLVILLE
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Subcontracting experience
1 record from government sources. Past participation is not evidence of current teaming interest.
| Role | Agency | Description | Vehicle | Obligated | Action date | Period |
|---|---|---|---|---|---|---|
| Subcontractor | Department of Health and Human Services | OPTION 10A
NASOPHARYNGEAL ASPIRATES WILL BE COLLECTED FROM CHILDREN ENROLLED IN THE UMOYA COHORT AT TYBERBERG HOSPITAL (STELLENBOSCH UNIVERSITY) IN CAPE TOWN, SOUTH AFRICA. THOSE SAMPLES TESTING POSITIVE FOR INFLUENZA VIRUS OR SARS-COV-2 WILL BE IDENTIFIED VIA MULTIPLEX RT-PCR. SARS-COV-2 POSITIVE SAMPLES WILL BE SENT FOR VIRUS ISOLATION AT THE DIVISION OF MEDICAL VIROLOGY, STELLENBOSCH UNIVERSITY (WHERE THE BSL3 LAB IS LOCATED, AND WHERE STAFF FROM THE SHAW LAB ARE ALSO WORKING). RNA WILL ALSO BE EXTRACTED FOR SEQUENCING. INFLUENZA POSITIVE SAMPLES WILL BE SENT TO THE SHAW LAB AT THE UNIVERSITY OF THE WESTERN CAPE FOR VIRUS ISOLATION, AND RNA EXTRACTION/CDNA AMPLIFICATION.
TITERED STOCKS WILL BE ESTABLISHED FOR EACH VIRUS ISOLATE WHICH WILL BE SEQUENCE VERIFIED AND CHARACTERIZED FOR GROWTH PROPERTIES. ANTIVIRAL SUSCEPTIBILITY TESTING WILL BE PERFORMED FOR INFLUENZA VIRUS ISOLATES. REPRESENTATIVE VIRUSES WILL BE SHIPPED TO ISMMS FOR DEPOSITION WITH BEI.
RNA FROM THE INFLUENZA OR SARS-COV-2 POSITIVE SAMPLES WILL BE ISOLATED AND FOLLOWING CDNA AMPLIFICATION, FULL LENGTH VIRAL GENOME SEQUENCES WILL BE DETERMINED BY NEXT GENERATION SEQUENCING METHODS. FULL LENGTH SEQUENCES WILL BE DEPOSITED IN PUBLIC DATABASES E.G. GISAID. PHYLOGENETIC ANALYSES WILL BE PERFORMED TO COMPARE THE GENETIC RELATIONSHIP OF THESE VIRUSES TO OTHER INFLUENZA VIRUSES OR SARS-COV-2 ISOLATES IN THE GLOBAL DATABASE.
FINALLY, FOR INFLUENZA VIRUSES, WE WILL DETERMINE THE GENETIC HETEROGENEITY OF THE QUASISPECIES IN A PATIENT BY CALCULATING THE INTRINSIC SEQUENCE DIVERSITY OF THE INFLUENZA VIRUS POPULATION IN EACH CLINICAL SAMPLE. 0258-A701-4612 Parent award context Parent award agency: Department of Health and Human Services · National Institutes of Health Parent award NAICS: 541715 Parent award performance state: NY Parent award description: MSSM CEIRR - THIS CONTRACT WILL SUPPORT THE NIAID CENTERS OF EXCELLENCE FOR INFLUENZA RESEARCH AND RESPONSE. View parent award on USAspending | — | $193,875 | Dec 14, 2021 | — – — |
Contract vehicles are shown only when present in the source record. Linked award numbers and vehicles open the authoritative record on USAspending.gov.
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