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THE WISTAR INSTITUTE OF ANATOMY AND BIOLOGY
PHILADELPHIA, PA, 19104
UnclaimedClaim this profile (free)
Subcontracting experience
3 records from government sources. Past participation is not evidence of current teaming interest.
| Role | Agency | Description | Vehicle | Obligated | Action date | Period |
|---|---|---|---|---|---|---|
| Subcontractor | Department of Health and Human Services | THE SUBCONTRACTOR WILL SHIP A DNA EP DELIVERY SYSTEM C2K DEVICE (+APPLICATORS) AND PROVIDE EXPERTISE, VIDEO, AND IN-PERSON TRAINING TO TULANE LAB MEMBERS IN THE USE OF THIS ELECTROPORATION DEVICE, AS WELL AS ADVICE AND GUIDANCE ON EXPERIMENTAL DESIGN TUL-HSC-562886-24/25 Parent award context Parent award agency: Department of Health and Human Services · National Institutes of Health Parent award NAICS: 541715 Parent award performance state: LA Parent award description: CONDUCT STUDY P223 View parent award on USAspending | 75N93020D00007USAspending | $62,130 | Jan 24, 2025 | — – — |
| Subcontractor | Department of Defense | SUBCONTRACTOR ON PROGRAM. H37810000708267 Parent award context Parent award agency: Department of Defense · Defense Contract Management Agency Parent award NAICS: 541715 Parent award performance state: NY Parent award description: RESEARCH IAW SOW - RAPID UNIVERSAL NUCLEIC ACIDS USING FIELDABLE AUTOMATED SYNTHESIS TECHNOLOGY (RUN FAST), DARPA NOW PROGRAM View parent award on USAspending | — | $420,658 | Nov 19, 2024 | — – — |
| Subcontractor | Department of Health and Human Services | OPTION 27A
STUDY OBJECTIVES AND SPECIFIC AIMS:
CRYO- AND NEGATIVE STAIN ELECTRON MICROSCOPY POLYCLONAL EPITOPE MAPPING (EMPEM) IS AN IMPORTANT NEW TECHNIQUE PIONEERED BY DR. ANDREW WARD (SEM-CIVIC INVESTIGATOR). NSEMPEM IS GREATLY EXPANDING OUR ABILITY TO UNDERSTAND THE POLYCLONAL RESPONSES AND DEFINE THE POPULATIONS OF ANTIBODIES TARGETING DIFFERENT EPITOPES INDUCED BY VACCINATION. TO PERFORM NSEMPEM, SAMPLES ARE PREPARED BY PURIFYING IGG ANTIBODIES FROM SERA, DIGESTING THE PURIFIED IGG ANTIBODIES INTO FABS WITH PAPAIN, AND COMPLEXING FABS WITH THE ANTIGEN. THE FAB-ANTIGEN COMPLEXES ARE IMAGED BY NEGATIVE STAIN ELECTRON MICROSCOPY. SINGLE PARTICLE EM DATA IS ANALYZED IN SOFTWARE PACKAGES TO PICK PARTICLES, PERFORM 2D CLASSIFICATION OF FAB POPULATIONS BY THE EPITOPE THEY TARGET, AND OBTAIN 3D RECONSTRUCTIONS OF FAB-ANTIGEN COMPLEXES. OUR LAB RECENTLY DEVELOPED AN AUTOMATED PIPELINE, €œCLOAKING WITH GLYCANS€Ͽ½ (CWG), TO SCREEN ALL RESIDUES FOR PNGS SEQUONS ON GLYCOPROTEINS. THIS PIPELINE USES ROSETTA, A PROTEIN DESIGN SOFTWARE. TO MODEL THE AMINO ACID MUTATIONS OF THE PNGS SEQUON AND THE ADDITION OF A GLYCAN TREE. ROSETTA SCORES FOR SOLVENT ACCESSIBLE SURFACE AREA OF RESIDUE BEING MUTATED TO ASPARAGINE, CLASHING INTRODUCED BY THE INTRODUCTION OF THE SEQUON, AND CLASHING OF THE GLYCAN WITH THE STRUCTURE ARE CALCULATED. CUTOFFS DETERMINED USING THE SCORES OF NATIVE NLINKED GLYCANS ON A PANEL OF VIRAL GLYCOPROTEINS ARE USED TO SELECT PNGS MODIFICATIONS TO SCREEN IN VITRO. THE CWG PIPELINE IS THE FIRST ROBUST, AUTOMATED METHOD FOR SCREENING FOR PNGS.
WE ARE INTERESTED IN EXPLORING HOW ANTIBODY SPECIFICITY CAN BE ALTERED BY INCORPORATION OF PNGS INTO INFLUENZA GLYCOPROTEINS. NSEMPEM WILL AFFORD THE ABILITY TO UNDERSTAND THE SHIFT IN THE POPULATION DISTRIBUTION OF VACCINE-INDUCED ANTIBODIES TOWARDS SPECIFIC EPITOPES AND INFORM AN ITERATIVE IMPROVEMENT OF IMMUNE FOCUSING TO SELECT EPITOPES IN WAYS WHICH OTHER ASSAYS ARE LIMITED. HERE, WE PROPOSE TO CONTRAST THE IMMUNODOMINANCE PATTERNS OF THE SERA FROM MICE IMMUNIZED WITH THE WT AND IMMUNE FOCUSED A/H1N1/NEW CALEDONIA/20/1999 NANOPARTICLE CONSTRUCTS THAT WE HAVE DEVELOPED TO DEFINE THE PATTERN OF TARGETED EPITOPES AND TO REFINE OUR CWG METHOD. TO ENSURE SUFFICIENT SERA, WE WILL POOL THE TERMINAL BLEED TIMEPOINT SERA THE MICE IN EACH GROUP (WT AND IMMUNE FOCUSED), PURIFY IGG ANTIBODIES USING A PROTEIN G COLUMN, DIGEST IGG INTO FABS USING PAPAIN DIGESTION KITS, COUPLE FABS TO NC99 HA TRIMERS, AND PURIFY COMPLEXES BY SIZE EXCLUSION CHROMATOGRAPHY. COMPLEXES WILL BE ADSORBED ONTO GLOW DISCHARGED CARBON-COATED COPPER EM GRIDS, STAINED WITH URANYL ACETATE, AND IMAGED WITH A FEI TECNAI T12 MICROSCOPE EQUIPPED WITH ONEVIEW GATAN CAMERA. WE WILL THEN BE TRAINED TO PROCESS THE SINGLE PARTICLE ANALYSIS TO BE ABLE TO CLASSIFY ANTIBODIES BY EPITOPES AND PERFORM 2D CLASS AVERAGES OF THESE POPULATIONS, AND PERFORM 3D RECONSTRUCTIONS. WE WILL HARNESS WHAT WE LEARN TO FURTHER DEVELOP OF OUR CWG PLATFORM, SO THE NUMBER AND DENSITY OF GLYCANS INCLUDED IN IMMUNOGENS WILL MORE LIKELY INDUCE THE ANTIBODY SPECIFICITY INTENDED. THESE STUDIES WILL HELP US TO BETTER CHARACTERIZE ANTIBODY SPECIFICITY, IMPROVE IMMUNE FOCUSING EFFORTS FOR INFLUENZA, AND INFORM IMPROVEMENTS ON OUR CWG PIPELINE FOR BROAD APPLICATION TO A RANGE OF VIRAL GLYCOPROTEINS.
FIT WITH CURRENT CIVICS PROJECTS:
THE CURRENT CIVIC PROGRAM IS FOCUSED ON DEVELOPING ENHANCED SEASONAL OR UNIVERSAL INFLUENZA VACCINES. MANIPULATING ANTIBODY RESPONSES BY IMMUNE FOCUSING CAN RESULT IN HIGHER LEVELS OF FUNCTIONAL, NEUTRALIZING ANTIBODIES AND POTENTIALLY RESULT IN TARGETING OF MORE CONSERVED EPITOPES. EMPEM IS A KEY METHOD USED WITHIN THE SEM-CIVIC TO UNDERSTAND RESPONSES AND WE SEEK TO APPLY EMPEM TO AID FURTHER DEVELOPMENT OF OUR IMMUNE FOCUSED NANOPARTICLE HA VACCINES. 0258-A460-4609 (DR. KULP) Parent award context Parent award agency: Department of Health and Human Services · National Institutes of Health Parent award NAICS: 541715 Parent award performance state: NY Parent award description: THIS CONTRACT WILL SUPPORT COMPONENT A OF THE COLLABORATIVE INFLUENZA VACCINE INNOVATION CENTERS (CIVICS) PROGRAM TO DESIGN AND EVALUATE INNOVATIVE INFLUENZA VACCINE APPROACHES, BASED ON PRINCIPLES OF INFLUENZA IMMUNITY, THAT ACHIEVE DURABLE, ROBUST View parent award on USAspending | — | $15,492 | Aug 18, 2022 | — – — |
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