← Back to search
LOW EARTH ORBIT BIOSCIENCES LLC
INDIAN HARBOUR BEACH, FL, 32937
UnclaimedClaim this profile (free)
Subcontracting experience
1 record from government sources. Past participation is not evidence of current teaming interest.
| Role | Agency | Description | Vehicle | Obligated | Action date | Period |
|---|---|---|---|---|---|---|
| Subcontractor | National Aeronautics and Space Administration | CONTRACT OVERVIEW
THIS CONTRACT IS REQUESTED FOR LOW EARTH ORBIT BIOSCIENCES TO PROVIDE MISSION INTEGRATION AND OPERATION SERVICES FOR MANIFEST AND LAUNCH OF THE PAYLOAD TO THE INTERNATIONAL SPACE STATION FOR THE INVESTIGATION TITLED: “SCALABLE COMMERCIAL BIOMANUFACTURING IN LOW EARTH ORBIT” FROM WILLIAM MCLAMB, PHD, AT LOW EARTH ORBIT BIOSCIENCES LLC.
PURPOSE
THIS STATEMENT OF WORK IDENTIFIES THE SCIENCE AND ENGINEERING REQUIREMENTS FOR CONDUCTING THE PROPOSED INVESTIGATION ON THE INTERNATIONAL SPACE STATION (ISS) NATIONAL LABORATORY AND IDENTIFIES THE MISSION INTEGRATION AND OPERATIONS SERVICES NEEDED FOR MANIFESTING AND LAUNCHING THE PAYLOAD TO THE ISS. THE INVESTIGATION IS TITLED: “SCALABLE COMMERCIAL BIOMANUFACTURING IN LOW EARTH ORBIT”.
OVERVIEW/EXPERIMENT SUMMARY
THE GOAL OF THIS PROJECT IS TO SUCCESSFULLY DEVELOP, INTEGRATE AND OPERATE SCALABLE BIOREACTOR TECHNOLOGY DEVICES ON ISS AND DEMONSTRATE MEASURABLE PERFORMANCE RESULTS. PI INTENDS TO DEMONSTRATE AND ADVANCE MULTIPLE NEW CAPABILITIES: 1) AUTOMATED CELL CULTURE, 2) PRECISION FLUID HANDLING, 3) FLUID/SAMPLE AGITATION, 4) IN SITU MEASUREMENT, 5) CRYOGENIC HANDLING AND 6) AUTOMATED CELL COUNTING.
THE PROPOSED OBJECTIVES FOR THE ISS PHASE ARE:
GOAL 2-0: DEMONSTRATE FEASIBILITY OF MANUFACTURING IPSCS USING SELECTED BIOREACTOR SYSTEM. ALTERNATIVE SUSPENSION CELL MODELS COULD BE USED THAT ARE ALSO SUITABLE FOR EXPANSION AND RELEVANT TO CELL AND GENE THERAPY. CELL MODELS WILL BE EVALUATED AND SELECTED DURING GROUND TESTING (AND COULD INCLUDE: SF9 CELLS, CD8+ T CELLS, HEK293 CELLS, AND MONOCYTIC CELL LINES SUCH AS THP-1). CELLS WILL BE THAWED INTO THE BIOREACTOR SYSTEM, PASSAGED AT LEAST 2 TIMES, AND THEN CRYOPRESERVED. CELLS WILL BE EVALUATED AT EACH PASSAGE USING THE KERMIT MICROSCOPE OR BIOSERVE INVERTED MICROSCOPE AND/OR PI-PROVIDED AUTOMATED CELL COUNTER. CRYOPRESERVED CELLS, FROZEN MEDIA AND FROZEN CELL PELLETS SAMPLES WILL BE TRANSPORTED BACK TO PI LAB FOR ASSESSMENT. THESE STUDIES WILL INCLUDE THE USE OF ANTIBIOTICS (PENICILLIN/STREPTOMYCIN) IN THE MEDIA.
GOAL 2-1: DEMONSTRATE FEASIBILITY OF PERFORMING ASEPTIC PROCESSES IN MICROGRAVITY. THE STUDIES IN GOAL 2-0 WILL BE REPEATED WITHOUT THE USE OF ANTIBIOTICS IN THE MEDIA.
PAGE 2 OF 6
GOAL 2-2: SHOW REPRODUCIBILITY OF EXPANSION OF IPSCS USING SELECTED BIOREACTOR SYSTEM IN MICROGRAVITY. EXPERIMENT IN GOAL 2-1 WILL BE REPEATED MULTIPLE TIMES INCLUDING MULTIPLE MANUFACTURING RUNS USING MULTIPLE RESEARCH AND CLINICAL IPSC LINES.
GOAL 2-3: SPACEFLIGHT CERTIFICATION OF COTS BIOREACTOR DEVICE, COTS AUTOMATED CELL THAWING DEVICE, COTS AUTOMATED CELL COUNT/VIABILITY DEVICE, AND COTS IN SITU MEASUREMENT DEVICE.
REQUIREMENTS
LOW EARTH ORBIT BIOSCIENCES WILL PERFORM DURING THE SCIENCE DEFINITION AND IMPLEMENTATION PHASES PER NATIONAL LAB AND ISS GUIDELINES FOR PAYLOADS AND HARDWARE TO SUPPORT ACTIVITIES DURING SCIENCE REQUIREMENT DEFINITION, SCIENCE VERIFICATION TEST (SVT), PAYLOAD MANIFEST, EXPERIMENT VALIDATION TEST (EVT), AND ALL MISSION OPERATIONS PREFLIGHT THROUGH POST-FLIGHT SAMPLE RECOVERY AND DELIVERY TO THE PRINCIPAL INVESTIGATOR (PI). THIS ALSO INCLUDES COORDINATING THE USE OF ANY FACILITIES NEEDED TO SUPPORT THE EXPERIMENT (E.G., PORTABLE GLOVE BAG AND COLD STOWAGE FACILITIES). LOW EARTH ORBIT BIOSCIENCES SHALL PERFORM ALL THE WORK NECESSARY FOR THE SUCCESSFUL INTEGRATION, LAUNCH, ON-ORBIT OPERATIONS, SAMPLE RETURN, AND GROUND CONTROL FOR THE FLIGHT EXPERIMENT.
REQUIRED HARDWARE
LOW EARTH ORBIT BIOSCIENCES LLC SHALL PROVIDE THE FOLLOWING HARDWARE:
•
BIOREACTOR PLATFORM (TO BE INSTALLED/OPERATED WITHIN BIOSERVE’S SABL)
•
CRYOGENIC THAWING SYSTEM
•
AUTOMATED CELL COUNTER
•
POWER SUPPLY (MODIFIED FOR CONNECTIVITY TO ISS POWER), IF NOT EXISTING
•
EXPERIMENT UNIQUE EQUIPMENT (EUE) HARDWARE FOR BOTH FLIGHT AND GROUND CONTROLS LAUNCHED AT AMBIENT, INCLUDING:
O
SEEDING KITS LAUNCH (FROZEN CELLS, GROWTH FACTORS) LAUNCHED AT <-80ºC
O
MEDIA EXCHANGE KITS, LAUNCHED AT <-20ºC
O
FLUID/SAMPLE TRANSFER KITS, LAUNCHED AT AMBIENT
•
TH 1567500 · Prime awardUSAspending | 80JSC025DA003USAspending | $82,500 | Mar 24, 2026 | — – — |
Contract vehicles are shown only when present in the source record. Linked award numbers and vehicles open the authoritative record on USAspending.gov.
Government-sourced data comes from SAM.gov, SBA and USAspending.gov and is attributed to its source. Items marked company-reported were provided by the company and are verified by FedSub only where labeled. FedSub is a private company and is not affiliated with SBA, GSA/SAM.gov, or any government agency.