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KAROLINSKA INSTITUTET
SOLNA, 17165
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1 record from government sources. Past participation is not evidence of current teaming interest.
| Role | Agency | Description | Vehicle | Obligated | Action date | Period |
|---|---|---|---|---|---|---|
| Subcontractor | Department of Health and Human Services | OPTION 22B: 0258-A728-4612
TOWARDS UNIFIED QUANTITATIVE ASSAYS OF MUCOSAL IMMUNITY
MUCOSAL ANTIBODIES ARE CRITICAL FOR PROTECTION FROM SARS-COV-2 INFECTION. THE FIELD IS CURRENTLY USING DIFFERENT COLLECTION METHODS AND ASSAYS TO QUANTIFY MUCOSAL SARS-COV-2 ANTIBODIES. PUTTING TOGETHER A SET OF MATCHED SALIVA AND NASAL SECRETION SAMPLES THAT CAN BE USED BY A LARGER GROUP OF INVESTIGATORS, AS WELL AS BY NIAID, TO TEST AND BENCHMARK THEIR MUCOSAL ANTIBODY ASSAYS WOULD GREATLY ADVANCE THE FIELD. MOREOVER, IT WOULD INFORM ON THE STRENGTHS AND WEAKNESSES OF EACH COLLECTION METHOD; INFORMATION, WHICH IS CRITICAL FOR THE DEVELOPMENT OF STUDY PROTOCOL FOR LARGE-SCALE MUCOSAL VACCINE STUDIES.
DRS. KRAMMER AND SIMON DISCUSSED AND DEVELOPED THE FOLLOWING INTEGRATED TWO SPECIFIC AIMS WITH THEIR COLLEAGUES DRS. GOMMERMAN (UNIVERSITY OF TORONTO, CANADA) AND THÃƑÂ¥LIN (KAROLINSKA INSTITUTE, SWEDEN). EACH OF THE THREE SITES HAS BANKED BIOSPECIMEN AND ONGOING COLLECTION PROTOCOLS SUITABLE TO IMPLEMENT TO PROPOSED COLLECTIONS AND EVALUATIONS.
SPECIFIC AIM 1: TO SHARE EXISTING BANKED SALIVA, NASAL AND SERUM SAMPLES TO GENERATE BIOSPECIMEN POOLS TO BE DISTRIBUTED TO SAVE LABORATORIES
DR. THÃƑÂ¥LIN€„¢S LAB AT KAROLINSKA INSTITUTET WILL BANK MATCHED NASAL SECRETION (ANTE NARES SWAB), SALIVA (DROOLING) AND SERA (VENIPUNCTURE) FROM 20 PARTICIPANTS.
SPECIFIC AIM 2: TO COLLECT SALIVA AND NASAL SECRETIONS FOLLOWING HARMONIZED PROTOCOLS TO MEASURE THE QUANTITY OF MUCOSAL SARS-COV-2 ANTIBODY (SUBTYPES, SECRETED IGA).
NASAL SECRETIONS, SALIVA AND SERA WILL BE PROVIDED FROM 20 PARTICIPANTS TWICE A YEAR. THE SAMPLES WILL BE SHARED BETWEEN THE THREE PARTNERING LABS TO COMPARE AND MEASURE SARS-COV-2 ANTIBODIES WITH EACH LABS UNIQUE TESTING PLATFORM.
METHODS
NASAL SECRETIONS: LEFT AND RIGHT ANTE NARES WILL EACH BE SWABBED WITH A COTTON SWAB WHICH WILL BE RESUSPENDED IN 1ML PBS (YIELD: 2ML TOTAL FOR BOTH NOSTRILS).
SALIVA WILL BE COLLECTED FROM THE SAME PARTICIPANT AT THE SAME VISIT USING THREE DIFFERENT PROTOCOLS: PASSIVE DROOLING (YIELD: 2ML SALIVA), SALIVETTE (YIELD: 1ML SALIVA) AND DIPPING OF A COTTON SWAB FOLLOWED BY RESUSPENSION IN 0.5ML PBS. OF NOTE, THE COMPLEXITY OF THE COLLECTION METHOD IS INVERSELY CORRELATED WITH THE EASE OF COLLECTION AS WELL AS THE VOLUME OF SPECIMEN COLLECTED (E.G., THE EASIEST WAY TO COLLECT SALIVA IS TO USE A COTTON SWAB BUT THIS METHOD ALSO PROVIDES THE SMALLEST AMOUNT OF SPECIMEN).
SERUM: 3-5ML SERUM WILL BE COLLECTED BY VENIPUNCTURE AT THE SAME STUDY VISIT.
APPROACH
THE THÃƑÂ¥LIN LAB WILL COLLECT AND SHARE WITH BEI RESOURCES PAIRED BLOOD, NASAL AND SALIVA SAMPLES FROM A TOTAL OF 40 STUDY PARTICIPANTS (HIGH AND LOW MUCOSAL ANTIBODY LEVELS), WHICH CAN THEN BE ACCESSED BY SAVE INVESTIGATORS AND SELECTED NIAID INVESTIGATORS FOR CONCORDANCE TESTING. DATA ON THE SAMPLES FROM THE THÃƑÂ¥LIN LAB WILL BE COMBINED WITH DATA FROM THE GOMMERMAN, KRAMMER AND SIMON LABORATORIES AND WILL FORM THE BASIS FOR A MANUSCRIPT DESCRIBING CONCORDANCE BETWEEN METHODS AND CORRELATIONS OF MUCOSAL ANTIBODIES IN DIFFERENT SAMPLES. 0258-A728-4612 Parent award context Parent award agency: Department of Health and Human Services · National Institutes of Health Parent award NAICS: 541715 Parent award performance state: NY Parent award description: MSSM CEIRR - THIS CONTRACT WILL SUPPORT THE NIAID CENTERS OF EXCELLENCE FOR INFLUENZA RESEARCH AND RESPONSE. View parent award on USAspending | — | $72,500 | Apr 18, 2024 | — – — |
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